Job Description Summary:
The Associate Scientist II, Biology (Cell Biology) is responsible for performing scientific experiments that contribute to research and drug discovery activities at PTC. This position assists in the conduct of early stage research, the goal of which is the identification of New Chemical Entities (NCEs), as well as the advancement of research programs from the identification of screening hits to advanced lead optimization. The incumbent is also responsible for communicating experimental results to his/her supervisor and the project team.
The Associate Scientist II, Biology supports adherence to all regulatory requirements and company Standard Operating Procedures (SOPs) including as appropriate.
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Job Description:
ESSENTIAL FUNCTIONS Primary duties/responsibilities:
Develop innovative induced pluripotent stem cell (iPSC)-based models of neurological diseases using patient-derived fibroblasts and genome-edited cell lines. Contribute to the development and optimization of in vitro differentiation protocols, facilitating the production of iPSC-derived cell types, especially neurons. Perform neural/glial differentiations for phenotype discovery. Support hit validation and hit-to-lead optimization by screening compounds and/or supporting structure activity relationship (SAR) studies involving the characterization of the biological activity of compounds in various assays. Provide support for high throughput screening including the design of primary assays to identify hits and new chemical scaffolds. Execute experiments under minimal supervision and communicate results within multi-disciplinary team. May design and conduct independent research experiments. Analyze experimental data using tools like R, Prism, Excel, etc. Apply relevant scientific principles and techniques to research problems. Maintain broad knowledge of state-of-the-art principles and techniques, contributing to the identification of new project areas or technologies. Present findings internally and externally contributing to publications in peer-reviewed journals, subject to internal scientific and legal approval. Maintain detailed electronic laboratory notebooks. Provide technical and scientific input to the project team or research leadership. Comply with all safety requirements. Contributes to general lab operations such as:
Equipment maintenance. Identification of capital equipment needs. Maintenance of clean and uncluttered work areas. Maintain Inventory and order supplies as needed.
KNOWLEDGE/SKILLS/ABILITIES REQUIRED * Minimum level of education and years of relevant work experience.
Bachelor's degree in a scientific discipline with 5 years' experience or Master's degree in a scientific discipline with over 2 years of relevant experience. Experience (academic or industry) in cell culture required. Proficient in iPSC maintenance, reprogramming, and differentiation techniques using various protocols.
* Special knowledge or skills and/or licenses or certificates required.
Hands-on experience culturing iPSCs, glial cells, and neurons. Experience in human iPSC/ESC culture, iPSC reprogramming, and CRISPR/Cas9 genome editing is highly desired. Ability to work productively in a dynamic and fast-paced work environment. Strong technical proficiency, scientific creativity, independent thought, and collaborative skills in a cross-functional team environment. Knowledge of scientific principles and current relevant scientific literature. Analytical thinking and problem-solving skills with adaptability to changing priorities and deadlines. Effective planning, organization, and time management skills with ability to support and prioritize multiple projects. Proficiency with Microsoft Office, MS Excel, MS PowerPoint, Graphpad Prism. Effective verbal and written communication skills.
*Special knowledge or skills and/or licenses or certificates preferred.
Background in neurobiology, stem cell biology, genome editing, cell-based assay development, laboratory automation, or high-content cell analysis. Proficiency in flow cytometry is preferred. Hands-on experience with mammalian cell culture including DNA/RNA transfection, Lentiviral transduction, isolation of stable clones, cryopreservation, RNAi/CRISPR, cytotoxicity, cell-based reporter assays (e.g., luciferase assays, protein-protein interaction) and compound screening in 96- and/or 384-well format. Experience with RNA purification and gene expression quantification (e.g., ddPCR, PCR and qRT-PCR). Familiarity with molecular cloning, SDS PAGE, western blot, ELISA/Mesoscale Discovery (MSD). Experience in high-throughput cell-based assays, laboratory automation and robotic plate handlers (e.g. JANUS, Assistant Plus). Experience with RNA library preparation and Next Generation Sequencing.
* Travel requirements
*Physical requirements
Office/Laboratory based-position, full-time on-site Mon-Fri. Flexible scheduling, including occasional weekend work is required to maintain healthy cell cultures.
. EEO Statement: PTC Therapeutics is an equal opportunity employer. We welcome applications from all individuals, regardless of race, color, national origin, gender, age, physical characteristics, social origin, disability, religion, family status, pregnancy, sexual orientation, gender identity, gender expression, disability, veteran status or any unlawful criterion under applicable law. We are committed to treating all applicants fairly and avoiding discrimination. Click here to return to the careers page
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PTC Therapeutics, Inc
Feb 27, 2025